TABLE 1.

Steady-state ATPase kinetics for RecD2

All measurements were at 20 °C in the presence 10 μm MDCC-PBP, 1.5 nm RecD2, and 500 nm dT₂₀ in a buffer described under “Experimental Procedures.”

Nucleotide	kcat	Km
	s−1	μm
ATP	86.9 ± 1.5	30.3 ± 1.5
ATP (no DNA)	1.6 ± 0.3	9.4 ± 2.5
MantATP	37.3 ± 1.4	9.7 ± 1.3
MantATP (no DNA)	0.5 ± 0.1	7.8 ± 2.2
Mant-deoxyATP	40.2 ± 2.4	12.1 ± 1.7
MantATPγS	0.3 ± 0.1	8.2 ± 1.9