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Suv39h1ΔN did not interact with HP1. A, Suv39h1, HP1, and Dnmt3 constructs were used in this study. B, co-immunoprecipitation experiments for Suv39h1 and HP1. FLAG-tagged WT Suv39h1, ΔN, or H324L were co-transfected with Myc-tagged HP1α, -β, or -γ into HEK293T cells. After 48 h of transfection, the extracted cell lysates were immunoprecipitated (IP) with anti-FLAG or anti-Myc antibodies and then subjected to Western blot analysis with anti-FLAG or anti-Myc antibodies.
