TABLE 3.

Potencies of PeIA peptides with multiple substitutions on rat nAChRs expressed in Xenopus oocytes

Peptide	α3β2			α6/α3β2β3
	IC50	95% C.I.	IC50 ratio relative to PeIAa	IC50	95% C.I.	IC50 ratio relative to PeIAa
PeIA	19.2 nm	17.2–21.5	0	17.2 nm	15.1–19.5	0
PeIA(S9H,V10A,N11R)	34.0 nm	31.3–37.4	0.2	1.48 nm	1.18–1.16	−1.1
PeIA(A7V,S9H,V10A,N11R)	10.1 μm	6.90–17.1	2.7	2.53 nm	2.14–2.98	−0.8
PeIA(S9H,V10A,N11R,E14A)	106 nm	79.2–142	0.7	4.91 nm	4.28–5.61	−0.5
PeIA(A7V,S9H,V10A,N11R,E14A)	30.9 μm	22.8–42.1	3.2	2.16 nm	1.98–2.35	−0.9

^a Indicates the log change in the ratio of IC₅₀ values relative to PeIA. Negative values indicate an increase in potency, whereas positive values indicate a decrease in potency.