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Effects of MAPK inhibitors on AP-1 DNA binding to IL-8 promoter. H441 cells were first incubated with PD98059 (PD) (30 μm), SB203580 (SB) (10 μm), or SP600125 (SP) (10 μm) for 1 h and then treated with ESAT-6 (5 μg/ml) for 3 h (A) or 6 h (B). Nuclear proteins were isolated, and AP-1 binding to IL-8 promoter was determined by an electrophoretic mobility shift assay.
