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. 2013 Jul 18;288(35):25500–25511. doi: 10.1074/jbc.M112.448217

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — ESAT-6 induces formation of inflammatory cell aggregates or early granuloma formation in mouse lungs. Light micrograph sections of lungs of mice 3 days after administration of 40 μg of ESAT-6 or CFP10. Control mice received HBSS only. A, hematoxylin and eosin staining. a and d, control lung. b and e, lung from CFP-10-treated mouse. c and f, lung from ESAT-6-treated mouse. Magnification was ×40 (a–c) and ×400 (d–f). B, KC immunostaining. a, control; b, lung from CFP-10-treated mouse; c, lung from ESAT-6-treated mouse; d, lung from ESAT-6-treated mouse showing cell aggregate. Staining in alveolar type II (arrow) and bronchiolar (arrowhead) epithelial cells and macrophages (open arrow) are shown. Enlarged sections of original photomicrographs (×200) are shown.