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LC-MS of B. subtilis xylanase A expressed in P. pastoris before (A) and after (B) Endo H digestion. Intact mass analyses indicate three main glycoform clusters with different extents of glycosylation. These data are supported by Endo H digestion, where the protein mass (24,050 Da) plus four, three, or two HexNAc monosaccharides was observed. The inset shows SDS-PAGE from purified xylanase before and after Endo H digestion.
