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Thermal inactivation profiles at 55 °C of the wild type and the mutants. A, wild-type xylanase A expressed either in E. coli (Wild-typeEc) or P. pastoris (Wild-typePp) and single mutants expressed in P. pastoris (see inset). B, results for double, triple, quadruple, and quintuple mutants. Error bars, S.D.
