FIGURE 4.
Shown is a hydrogen/deuterium exchange study on the free (A) and PmrD-bound (B) PmrAN. The overlay of 1H,15N TROSY-HSQC spectra of protein samples incubated in 90% H2O, 10% D2O (black) or in 100% D2O for 5 min (red). Three PmrAN residues, which are water-inaccessible in the bound form only, are labeled in blue. C, ribbon representation of PmrAN monomer. The residues that are water-inaccessible in the free PmrAN are colored in blue, and the three residues that were water-inaccessible only in the PmrD-bound PmrAN are colored and labeled in red.