TABLE 1.

Kinetic parameters for yeast tRNA^Phe transcript

Although the results of gel mobility shift assays were evaluated by 4° by the intensities of shift bands, the differences except for D190A were small as described in the text.

Variant name	Km	Vmax	Vmax/Km	Gel mobility shift assay
	nm	μmol mg−1 h−1
Wild type	150	1.1	1.00	++
D180A	120	0.4	0.42	++
E182A	110	0.2	0.29	++
Y184A	260	0.2	0.10	+
Q185A	120	0.1	0.16	±
K186A	160	0.1	0.07	±
V187A	140	0.1	0.11	±
D190A	1300	0.2	0.05	−