FIGURE 6.

Human PtGR-1 overexpression increases cellular nitroalkene reductase activity. A, HEK293T cells were transfected either with an empty or a FLAG-tagged human PtGR-1 expression vector. Recombinant FLAG-hPtGR-1 migrates at ∼40 kDa (shown via anti-FLAG immunoblotting (IB)), and endogenous PtGR-1 was detected at 36kDa. B–E, time-dependent generation of reduced NO₂-OA metabolites in HEK293T growth media upon cell transfection with either empty vector or human PtGR-1 gene. The initial NO₂-OA was 15 μm. Data are the means ±S.E. n = 3. hPtGR-1 transfection increases the generation of the reduced 18-, 16-, 14-, and 12-carbon metabolites of NO₂-OA as determined by two-way ANOVA and Bonferroni post tests. *, p < 0.01.