Skip to main content
. 2013 Jun 11;4(6):911–922. doi: 10.18632/oncotarget.1066

Figure 1. Caspase-dependent apoptosis is not the only form of active cell death occurring in OHT-treated HMCLs.

Figure 1

A) Exponentially growing RPMI 8226 and LP-1 cells were treated with EtOH for control or OHT (10 μM for 72 h) and apoptotic cells were analyzed after APO2.7 staining and cytometry sorting. The percentage of apoptotic cells (APO2.7-stained) is indicated on the graph. B) LP-1 cells were treated with vehicle or 10 μM OHT for 48 h and stained with DiOC6(3) before cytometry sorting. For cytometry analyses, an average of 2 × 105 cells was sorted for each culture condition. C) RPMI 8226 and LP-1 cells were cultured with (or without) 10 μM Q-VD-OPh for 2 h, then treated with 10 μM OHT for 48 h. The number of viable cells was then determined with a MTT assay. Each experimental condition was repeated five times; the experiment was repeated twice. Plotted values are the percentage of viable cells referring to control experiments assigned to 100%. *, p<0.05.