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. 2013 Jun 11;4(6):911–922. doi: 10.18632/oncotarget.1066

Figure 4. AEBS ligands induce PI3K/AKT/mTOR-mediated autophagy in HMCLs.

Figure 4

A) HMCLs were treated with RU 39411 (10 μM) or PBPE (40 μM) for 24 h and analyzed by transmission electron microscopy. B) and C) RPMI 8226 and LP-1 cells were cultured with (or without) 1 μM LY 294002 for 2 h (B), or 10 nM everolimus (RAD 001) for 2 h (C) then treated (or not) with 10 μM RU 39411 or 40 μM PBPE for 24 h (B) or 48 h (C). The number of viable cells was then determined with a MTT assay. Each experimental condition was repeated five times; the experiment was repeated three times. Plotted values (mean ± SD) are the percentage of viable cells referring to control experiments assigned to 100%. * p<0.05; ns, not significant.