Table 2.

Ethanol (EtOH) Concentration-Dependent Expression of Neurotransmitter Receptor Genes in the Spleen of HIV-1Tg Rats

	Normal (F344) rats: up-down regulation				HIV-1Tg rats: up-down regulation
Spleen	8% EtOH		52% EtOH		8% EtOH		52% EtOH
Gene symbol	Fold regulation	p-Value	Fold regulation	p-Value	Fold regulation	p-Value	Fold regulation	p-Value
Gabra1	2.2125	0.325	−1.1788	0.644144	1.5898	0.035334	1.8752	0.026759
Gabra2	1.6383	0.963101	1.6128	0.866792	1.4158	0.455336	2.0846	0.009679
Gabra3	1.2467	0.485339	−1.6778	0.227888	2.3792	0.259119	5.4542	0.009679
Gabra4	2.1235	0.185104	−1.0529	0.547259	1.8617	0.281836	1.8027	0.346848
Gabra5	1.8577	0.278528	−1.2036	0.337454	1.2171	0.393527	1.8027	0.132689
Gabra6	1.3617	0.372891	−4.3087	0.014536	1.9397	0.181173	2.0936	0.098364
Grm1	2.1235	0.185104	−1.0529	0.547259	1.3789	0.328842	1.8027	0.009679
Grm2	2.1235	0.185104	−1.0529	0.547259	1.2171	0.393527	1.8027	0.009679
Grm5	2.0689	0.145467	−1.5056	0.434396	1.2029	0.639434	1.5163	0.253526
Drd1a	9.4615	0.161875	1.9185	0.18737	1.2256	0.397125	1.9516	0.003872
Drd2	−1.455	0.726711	−2.4636	0.036113	2.3164	0.320178	2.636	0.018461
Drd3	1.678	0.425532	−2.0507	0.071218	−1.3218	0.667884	2.1714	0.035732
Drd4	3.2467	0.137367	3.9139	0.014791	−1.1266	0.376632	−1.1026	0.436736
Drd5	2.0365	0.220021	−1.0607	0.52967	−1.1398	0.950395	1.7879	0.326432

Significance determined at p < 0.05.

Neurotransmitter receptor (GABA_A receptor [Gabra 1 to 6], metabotropic glutamate receptor [Grm 1, 2, and 5], and dopamine receptors [Drd1a, 2 to 5]) gene expression was measured in the spleen of young adult HIV-1Tg and F344 normal rats treated with 0% (water control), 8% EtOH, or 52% EtOH in a 3-day binge regimen (total dose of 2.0 g/kg/d), using a custom-designed 48-gene PCR array. The fold change was calculated using the ΔΔCT method relative to the 0% EtOH group (n = 3 to 4 rats for each group) for each strain (F344 or HIV-1Tg).