Figure 3.

Phenotype of R482Q and Fbxw7^+/− E18.5 lungs. (A) H&E staining on E18.5 lung samples. Left panel: wild-type; middle panel: R482Q; right panel: Fbxw7 null heterozygotes (Fbxw7^+/−). Scale bars are 150 µm. The R482Q lungs have thickened alveolar septa, whereas the wild-type and Fbxw7^+/− have normal-thickness alveolar septa. The graph shows a summary of the septal thickness in wild-type and R482Q E18.5 lungs. (B) Ki67 immunohistochemistry staining of wild-type (left panel) and R482Q (middle panel) E18.5 lungs. Scale bars are 150 µm. There is an increase in the percentage of Ki67-positive proliferating cells in the R482Q sample shown in the graph in the right panel (p = 0.0275, t-test). Error bars represent the SEM