Fig. 4.

Myeloid cell dynamics are inhibited in injured Mmp12^−/− mouse corneas. (A) Examples of migration of myeloid cells in the stroma of injured corneas – a high-migratory cell in a WT cornea (yellow arrow), and a low-migratory cell in a Mmp12^−/− cornea (yellow arrow). See supplementary material Movies 1 and 2 for time-lapse recordings. Scale bars: 50 µm. (B) Representative tracts of migrating cells during 30 minutes of imaging. Each color represents one cell. (C) Motility analysis of myeloid cells showing average track lengths, average track lengths per minute, total displacement and velocity. (D) One day after injury, WT corneas have a greater proportion of c-fms-EGFP⁺ cells that are Gr-1⁺. Representative whole-mount micrographs show that most of the c-fms-EGFP⁺ cells are also positive for Gr-1 in WT corneas, whereas fewer are positive for Gr-1 in Mmp12^−/− corneas. (E) Injured WT corneas have a higher percentage of Gr-1⁺ cells compared with Mmp12^−/− corneas. The means ± s.e.m. percentage of Gr-1+ cells are shown and are 94±2.3% (WT; n = 9) and 64±13% (Mmp12^−/−; n = 8), *P<0.05. Scale bars: 100 µm.