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. Author manuscript; available in PMC: 2013 Aug 30.
Published in final edited form as: Cancer Res. 2007 Apr 15;67(8):3862–3870. doi: 10.1158/0008-5472.CAN-06-4282

Figure 4.

Figure 4

Reintroduction of the p53 wt gene in p53-null cells causes a complete restoration of the apoptotic response. p53-null cells were infected with an adenovirus (Ad) encoding the wild-type p53 gene and were subjected to noscapine exposure for 0, 12, 24, and 48 h. An empty vector control was used in the experiments to null the background effects of the adenovirus vector. A, three-dimensional FACS profile of Adp53-infected (I) and empty vector–infected (EV) cells for 0, 12, 24, and 48 h. B, quantitation of the percent sub-G1 population for Adp53-infected and empty vector–infected p53-null cells. C, graphical representation of the extent of apoptosis in Adp53-infected cells and empty vector–infected controls as evaluated by Annexin V staining followed by FACS analysis. Columns, average of three independent experiments; bars, SD (P < 0.05). D, immunoblot analysis of cell lysates from Adp53-infected and empty vector–infected p53-null cells.