Table 1.

Clinical Studies on the Impact of Regulatory T Cells in Disease Course and Outcome of Colorectal Cancer.

Author/Journal/Year	Patients Included	Method	Compartment	Major Conclusions
Loddenkemper et al. Journal of Translational Medicine 2006 [110]	40	IHC	Primary CRC	(1)No difference in survival in patients with high and low total FOXP3 infiltration (2)Weakly better survival in patients with higher epithelial Treg infiltration
Salama et al. Journal of Clinical Oncology 2009 [120]	967	IHC	Primary CRC	(1)Intratumoral FOXP3 density associated with better survival (2)High FOXP3 infiltration of normal mucosa associated with worse survival
Sinicrope et al. Gastroenterology 2009 [117]	160	IHC	Primary CRC	(1)Low intraepithelial CD3/FOXP3 is a independent marker for reduced 5-year DFS (2)CD3/FOXP3 stronger prognostic variable than tumor stage and LN number
Suzuki et al. Cancer Immunology and Immunotherapy 2010 [124]	95	IHC	Primary CRC	(1)High intratumoral CD8/FOXP3 independent prognostic marker for OS in patients undergoing curative surgery (2)Tumor TGF-β is implicated in FOXP3+ cell accumulation
Frey et al. International Journal of Cancer 2010 [122]	1420	IHC	Primary CRC	(1)High FOXP3 infiltration independent positive predictor for 5-year DSS in MMR-proficient patients (2)FOXP3 density not of prognostic value for MMR-deficient patients
Correale et al. Journal of Immunotherapy 2010 [123]	57	IHC	Primary CRC	(1)High FOXP3 infiltration associated with better OS (2)High FOXP3 infiltration predicted better treatment response (GOLFIG-2/FOLFOX-4)
Deng et al. Clinical Cancer Research 2010 [125]	34	FACS	Primary CRC/PB/MLN	(1)Proportion of Tregs in TDLN were > PB and < TIL (2)Infiltration of FOXP3+ cells correlated with disease stage
Matera et al. Gut 2010 [126]	30	IHC	MLN	(1)High FOXP3 levels in SLN associated with early disease stage (2)Better survival in patients with higher FOXP3 frequencies
Nosho et al. Journal of Pathology 2010 [118]	768	IHC	Primary CRC	(1)FOXP3 infiltration correlated with survival (2)FOXP3 is not a independent prognostic marker

Abbrevations: IHC: immunohistochemistry; CRC: colorectal cancer; Treg: regulatory T cells; DFS: disease free survival; LN: lymph nodes; OS: overall survival; MMR: mismatch repair; FACS: flow cytometry; PB: peripheral blood; MLN: mesenteric lymph node; TDLN: tumor draining lymph node; SLN: sentinel lymph node.