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. 2011 May 4;3(2):2274–2285. doi: 10.3390/cancers3022274

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Inhibitory effect of Tetrandrine on mammosphere formation. SUM-149 (A) and SUM-159 (B) cells were cultured in mammosphere-forming conditions. SUM-149 and SUM-159 cells were incubated with indicated dose of tetrandrine or DMSO in mammosphere formation media for 7 days. Tetrandrine treatment reduced the number of primary mammospheres in a dose dependent manner; (C) Representative image of mammospheres from SUM-159. Scale bar = 200 μM. The results are representative of three independent experiments; asterisks indicate P < 0.05 compared to control in all cases. Error bars represent standard deviation.