Table 5. Live adherent cell density of keratinocytes co-cultured with fibroblasts on sIPN, PEG hydrogel and PC surfaces.
| Surface | Exogenous KGF | Live adherent keratinocyte density (cells/mm2) after | |||
|---|---|---|---|---|---|
|
| |||||
| 2 h | 24 h | 96 h | 168 h | ||
| MPEG-sIPN | No | 1±1 | 0±0 | 0±1 | 6±6 |
| Yes | 1±1 | 0±0 | 1±1 | 3±1 | |
| GGG-sIPN | No | 1±1 | 5±7 | 0±0 | 2±2 |
| Yes | 0±0 | 0±0 | 1±1 | 3±3 | |
| RGD-sIPN | No | 5±2a,b | 4±4 | 0±0 | 1±1 |
| Yes | 1±1 | 1±0 | 1±1 | 4±5 | |
| PHSRN-sIPN | No | 3±0a,b | 16±6a,c | 10±16 | 10±4 |
| Yes | 0±0 | 1±2 | 1±2 | 4±5 | |
| Gelatin-sIPN | No | 0±0 | 3±1 | 0±1 | 6±6 |
| Yes | 1±1 | 2±1 | 0±0 | 0±0 | |
| PEG hydrogel | No | 12±5d | 5±3 | 5±2 | 25±14 |
| Yes | 7±8 | 6±10 | 6±10 | 1±1 | |
| PC (keratinocyte co-culture) | No | 12±7d | 11±7 | 25±17e | 204±95d |
| Yes | 13±8f | 15±4f | 89±105f | 156±94f | |
| PC (keratinocyte monoculture) | No | 7±0 | 33±26d | 78±21d | 332±184d |
| Yes | 12±6f | 14±6f | 82±12f | 236±153f | |
a
P < 0.05 vs. same substrate with exogenous KGF at the same time point.
b
P < 0.05 vs. sIPN control substrates prepared without exogenous KGF.
c
P < 0.05 vs. all other sIPN substrates prepared without exogenous KGF.
d
P < 0.05 vs. sIPN substrates prepared without exogenous KGF at the same time point.
e
P < 0.05 vs. keratinocyte monoculture without exogenous KGF.
f
P < 0.05 vs. sIPN substrates prepared with exogenous KGF at the same time point.