Table 7. IL-6 concentration in supernatant from keratinocytes co-cultured with fibroblasts on sIPN, PEG hydrogel and PC surfaces.
| Surface | Chamber | Exogenous KGF | IL-6 concentration (pg/ml) after | |||
|---|---|---|---|---|---|---|
|
| ||||||
| 2 h | 24 h | 96 h | 168 h | |||
| Co-culture mPEG-sIPN | A | No | 0±0 | 0±0 | 0±0 | 0±2 |
| B | No | 12±13 | 51±96 | 1±1 | 1±4 | |
| A | Yes | 0±0a | 0±0 | 161±183 | 161±183 | |
| B | Yes | 8±3 | 0±0 | 0±0 | 0±0 | |
| GGG-sIPN | A | No | 0±0 | 0±0 | 0±0 | 0±0 |
| B | No | 0±0 | 1±2 | 0±0 | 0±0 | |
| A | Yes | 0±0a | 0±1 | 0±0 | 0±0 | |
| B | Yes | 11±3 | 1±1 | 0±0 | 0±0 | |
| RGD-sIPN | A | No | 0±0 | 0±1 | 0±0 | 0±0 |
| B | No | 0±0 | 0±0 | 0±1 | 0±0 | |
| A | Yes | 0±0a | 1±1 | 0±0 | 0±0 | |
| B | Yes | 9±4 | 1±0 | 1±1 | 1±1 | |
| PHSRN-sIPN | A | No | 0±0 | 137±22a,b,c | 210±456 | 210±456 |
| B | No | 0±0 | 274±66b,c | 9±12 | 9±12 | |
| A | Yes | 0±0a | 0±0 | 0±0 | 0±0 | |
| B | Yes | 9±3 | 0±0 | 0±1 | 0±1 | |
| Gelatin-sIPN | A | No | 0±0 | 0±0 | 157±331 | 157±331 |
| B | No | 0±0 | 0±0 | 3±1 | 3±2 | |
| A | Yes | 0±0a | 3±8 | 0±0 | 0±0 | |
| B | Yes | 6±1 | 14±24 | 0±0 | 0±0 | |
| PEG hydrogel | A | No | 6±4a,d | 37±16 | 53±2 | 191±83 |
| B | No | 92±80 | 69±26 | 72±94 | 262±144c | |
| A | Yes | 22±5a,e | 17±23 | 40±52 | 58±54 | |
| B | Yes | 132±29e | 62±33e | 31±15 | 58±36 | |
| Polycarbonate | A | No | 12±4a,d | 102±41 | 183±127 | 742±190d |
| B | No | 143±40d | 137±122 | 453±606 | 1066±722d | |
| A | Yes | 28±14a,e | 82±25 | 948±746e | 1901±930e | |
| B | Yes | 166±34e,f | 118±45e | 968±530e | 1935±779e | |
| Monoculture PC (keratinocytes) | A | No | 7±1a,d | 20±5b | 269±242 | 835±262d |
| B | No | 0±1 | 14±1b | 173±30 | 793±164b,d | |
| A | Yes | 9±4a | 9±4 | 94±64 | 725±844 | |
| B | Yes | 1±1 | 6±2 | 112±84 | 365±165 | |
| TCPS (fibroblasts) | No | 181±51 | 640±73 | 1297±908 | 3925±1242b | |
| Yes | 147±11 | 500±99 | 591±422 | 1009±427 | ||
Fibroblasts were grown to confluency on 24-well TCPS plates. Supernatant was collected in the absence of a polycarbonate insert.
a
P < 0.05 vs. bottom chamber of same substrate.
b
P < 0.05 vs. all other sIPN substrates prepared without exogenous KGF.
c
P < 0.05 vs. same substrate with exogenous KGF at the same time point.
d
P < 0.05 vs. sIPN substrates prepared without exogenous KGF at the same time point.
e
P < 0.05 vs. sIPN substrates prepared with exogenous KGF at the same time point.
f
P < 0.05 vs. keratinocyte monoculture with exogenous KGF (same chamber).