Fig. 6. Effect of lysine acetylation on HAT activities of PfGCN5 and PfMYST.

GFP-tagged PfGCN5 and PfMYST were immunoprecipitated from P. falciparum trophozoites cultured without or with the inhibitor mixture (TSA and nicotinamide). Blots with anti-GFP antibodies indicate approximately equal amounts of purified PfGCN5 or PfMYST used in the HAT assays. The overall acetylation levels of the purified proteins were estimated by Western blots with anti-acetyllysine antibodies. HAT activities of purified PfGCN5 and PfMYST were determined using core histones and nucleosomal histones.