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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Oct 10;51(47):11863–11866. doi: 10.1002/anie.201204946

Figure 1.

Figure 1

Chemical shifts of imino protons δH (highlighted in magenta in the A:U and G:C base-pair diagrams) versus one-bond scalar coupling constants 1JNH, for 2'-F RNA (black squares) and RNA (red dots) of sequence C1G2A3A4U5U6C7G8. The graph shows average values with standard deviations (vertical bars) based on six independent one-dimensional 15N-coupled 1H IPAP spectra of the imino region in 10% D2O/H2O at 5°C (note the reversed scale on the y-axis). Entries around 12 ppm are for the G2 base, and those at 13.5 and 14 ppm are for the U6 and U5 bases, respectively. The average difference in 1JNH between 2'-F RNA and RNA amounts to 0.8 ± 0.3.