Skip to main content
. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Oct 10;51(47):11863–11866. doi: 10.1002/anie.201204946

Figure 3.

Figure 3

Region of 1H, 13C HMQC spectra of (a) 5'-r(CGAAUUCG)-3' and (b) 5'-f(CGAAUUCG)-3', showing the isotope effect at 13C2 of adenosine residues due to deuterium/proton substitution at the imino H3 site. The individual spectra were recorded at natural abundance 13C in 50% D2O/H2O at 25°C at 14.1 T spectrometer (600 MHz 1H frequency). The x- and y-axes refer to the 1H and 13C nuclei, respectively. The DIE measurements (2hΔ13C2 = δ13C2{1H3} − δ13C2{2H3}) show that 2'-F RNA exhibits a larger isotope effect than RNA (see Table S1 in the Supporting Information).