Fig. 2.

B16-BL6 mouse melanoma cells are not sensitized to PA toxins by prior paclitaxel treatment. Cells were seeded in 96-well plates, allowed to grow overnight, and treated with paclitaxel at 0–7.5 ng/mL for 24 h before the addition varying concentrations of PA (solid markers) or PA-U2 (open markers) with 500 ng/mL LF. The cells were allowed to grow an additional 48 h before the addition of 0.5 mg/mL MTT for an additional 1 h before lysis. Percent survival is reported as the absorbance of treatment wells compared to a vehicle-only control. Data is presented as the mean of three independent experiments and error bars are standard error. For many points, error bars are too small to see