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Representative Western blot images and density results of PRCP protein levels in several hypothalamic areas including the ARC, VMH, DMH, LH, and CTX of fed and fasted mice. The density of PRCP protein (upper band) was first normalized to β-actin (lower band) using a NIH imageJ program, and then compared between fed and overnight fasted groups. *p<0.05; **p<0.01; ***p<0.001.
