Table 1.
LC–MS/MS analysis of peptides containing histidine residues involved in chelation of zinc and copper.
| Peptidea | Calculated mass |
−H2O2 |
+H2O2 |
||
|---|---|---|---|---|---|
| Observed massb | Modification | Observed mass | Modification | ||
| A159VVVH163AGEDDLGR171 (T) | 1336.67 | 1336.65 (99) | None | 1336.65 (99) | None |
| 1352.64 (51) | His163(OX) | ||||
| A80LEGFPTEPDSSSRAIH96VH98QF100 (C)c | 2324.12 | 2324.08 (55) | None | 2324.09 (55) | None |
| 2340.09 (30) | His98(OX) | ||||
| S104QGCESTGPH113YNPL117 (C) | 1559.67 | 1559.64 (50) | None | 1559.65 (57) | None |
| A118VPH121PQH124PGDFGNF128 (C) | 1518.70 | 1518.68 (34) | None | 1518.68 (41) | None |
a
Peptides were generated by using trypsin (T) or chymotrypsin (C).
b
Observed masses represent the molecular mass of detected and deconvoluted ions. The MASCOT score is given in parenthesis.
c
The sequence is given with an Asn89→Asp substitution due to the conversion of Asn to Asp upon deglycosylation using PNGaseF.