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Cos-1 cells were transiently transfected with mPPARγ2, mRXRα, or empty control (pTargeT) expression vectors. Nuclear protein extracts were isolated 48 h after transfection and expression levels of PPARγ and RXRα were determined by Western blot analysis. The blot was then stripped and reprobed for Actin. This Western blot is a representative of at least four separate experiments
