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. 2013 Feb 5;1(1):172–177. doi: 10.1016/j.redox.2013.01.001

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig. 1 — Establishment ofSod2hepatocyte-specific knockout. (A) Western blot for SOD2 demonstrating loss of SOD2 protein in Sod2^−/− (KO) livers compared to Sod2^L/L (Con) livers. (B and C) Quantification of significant reduction in Sod2 message (B) and SOD2 activity (C) in KO livers compared to control. (D) Immunohistochemical staining for SOD2 demonstrates pan-hepatocellular knockout in Sod2^−/− livers compared to control. (E) Representative in situ DHE staining demonstrating enhanced nuclear positivity in Sod2^−/− livers compared to control.