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Western Blot of HNE–BSA standards and plasma. Quantitative analysis of Western Blots of HNE-protein adducts in HNE–BSA standards and plasma (120 pmol/mg with nc-Ab; 1250 pmol/mg with c-Ab) and a representative Western Blot analysis of HNE–protein adducts. Plasma sample was diluted to 10 mg/ml and 20 μg of protein (standards and plasma) were loaded onto gels.
