Fig. 2.

Acrolein-dependent Trx oxidation depends on GSH status. HBE1 cells were pretreated with BSO (24 h) or GEE (4 h) prior to acrolein treatment for 30 min, and Trx1 redox status was determined by S-carboxymethylation and non-reducing native PAGE. Representative redox Western blots are shown in (A) and relative band densities of partially oxidized Trx are summarized in (B). Results are expressed as mean±SEM, n=4. *: p<0.05, **: p<0.01 compared to control.