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Effects of TrxR1 alterations on acrolein-induced Trx1 oxidation/alkylation. HBE1 cells were supplemented with selenite or transfected with TXNRD1 siRNA and treated with acrolein for 30 min, and the oxidation/alkylation status of Trx1 was determined by redox Western blot (A and C). Graphs show relative band densities of partially and/or fully oxidized Trx1 (B and D). Results are expressed as mean±SEM, n=4. *: p<0.05, **: p<0.01 non-supplemented or NT-siRNA controls.
