Fig. 5.

IL-1α promotes NF-κB activation and interacts with HAT p300 in a redox-dependent manner. (A) NF-κB luciferase activity of HT1080 fibrosarcoma cells expressing indicated antioxidants transiently transfected with an IL-1α expression construct. Graph represents the average of 3 independent experiments +/− SEM. Where indicated, saturating concentrations of recombinant IL-1 Receptor antagonist (IL-1Ra) was used (10 ug/ml) (B) Immunoprecipitation of histone acetyl transferase p300 in indicated stable HT1080 fibrosarcoma cells followed by IL-1α immunoblot. (C) Immunoprecipitation of histone acetyl transferase p300 in 293 T cells with overexpressed IL-1α +/− 50 µM H₂O₂ for 1 h. Data represents n≥3±SEM. *p≤0.05, **p≤0.01.