Table 1.
Main parameters for MS data acquisition.
| Analyte | Cone (V) | Collision (eV) | Transitions m/z | Internal standard | Retention time (min) |
|---|---|---|---|---|---|
| GS–NEM | 20 | 22 | 433.4>201.3 | Phe–Ala-D5 | 9.5 |
| GSSG | 20 | 22 | 613.2>355.2 | Methionine-D3 | 5.85 |
| CyS–NEM | 25 | 20 | 247.3>158.2 | Phe–Ala-D5 | 8.84 |
| γ-GC–NEM | 20 | 25 | 376>230.3 | Phe–Ala-D5 | 9.53 |
| Methionine-D3 | 20 | 22 | 153.2>63.8 | n.a. | 3.88 |
| Phenylalanine-D5 | 20 | 15 | 171.1>125.1 | n.a. | 9.07 |
GS–NEM, CyS–NEM and γ-GC–NEM means N-ethylmaleimide (NEM) covalently bonded through a thioester linkage to reduced glutathione (GSH), Cysteine (CySH) and gamma-glutamylcysteine (γ-GC) respectively.