Table 1.

Reduction levels of reporters and corresponding calculated rates of H₂O₂ production from site I_F and site III_Qo in the presence of different substrates and inhibitors. With each substrate, the redox states of NAD(P)H and cytochrome b₅₆₆ were measured in parallel with the measurements of overall rates of H₂O₂ production reported in Fig. 5. Eqs. (2) and (3) describing the calibration curves in Fig. 2 were used to calculate the rates of superoxide/H₂O₂ production from site I_F and site III_Qo from these redox measurements. Concentrations of substrates and inhibitors are given in Fig. 5. All rates are in pmol H₂O₂ min⁻¹ mg protein⁻¹. Data are means±SEM (n≥4). Error values on the calculated rates of H₂O₂ production represent the propagated error as described in Materials and methods and Ref. [21].

Substrates and inhibitors	Redox state of site IFreporter and calculated rate of superoxide/H2O2production by site IF		Redox state of site IIIQoreporter and calculated rate of superoxide/H2O2production by site IIIQo
	Reduced NAD(P)H (%)	Reported rate of H2O2 production	Reduced cytochrome b566 (%)	Reported rate of H2O2 production
Succinate	89±1	59±4	46±2	91±18
Succinate+rotenone	80±2	49±4	50±1	124±15
Glycerol 3-phosphate	59±3	34±6	29±2	27±7
Glycerol 3-phosphate+malonate	59±3	34±6	29±2	27±7
Glycerol 3-phosphate+malonate+rotenone	39±2	31±5	31±3	33±9
Palmitoylcarnitine+carnitine	80±1	49±4	42±3	72±18
Palmitoylcarnitine+carnitine+malonate	79±1	48±4	44±2	80±17
Glutamate+malate	85±7	54±9	38±5	54±18