Fig. 3.

Structural differences in the cerebral cortex of Met–Emx1 mice are age specific. (A–H) Representative MRI images at rostral (A–D, bregma = + 1.04 mm) and caudal (E–H, bregma = − 1.66 mm) levels. Panels A, B, E, and F were obtained from P30 mice, whereas C, D, G, and H were from adult mice. (I and J) The cross-sectional area of the cerebral cortex is plotted versus estimated bregma level for P30 (I) and adult (J) animals. The overall distributions of area are the same for control and Met–Emx1 mice at P30 (D = 0.1076, p = 0.067, K–S test). Yet, the distributions are significantly different in control and Met–Emx1 mice at adulthood (D = 0.2200, p < 0.001, K–S test). Arrows denote the levels for which the rostral (gray arrows) or caudal (black arrows) images are shown. Data represent n = 4 brains per genotype and age, points represent the mean areas with the error bars signifying the SEM.