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. 2013 Jun 4;1(1):46–52. doi: 10.1016/j.stemcr.2013.03.003

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The WNT3 Level in hESCs Correlates with the DE Differentiation Potential

(A) Different hESC lines have different capacities for DE differentiation. The efficiency of DE differentiation was measured by the percentage of CD184 and CD117 double-positive cells after 4 days of induction.

(B) WNT3 messenger RNA (mRNA) levels in undifferentiated hESCs correlate with their DE differentiation efficiency after 4 days of induction. HES3, H9.2, MEL-1 (MG3) cultured with feeder, HUES8 cultured with Matrigel-mTeSR1, and H9.1 and HES2 cultured under both conditions were used in the differentiation assays. Shown is one representative result of three independent experiments.

(C) Immunofluorescent staining images show a correlation between WNT3 levels in hESCs and SOX17 or FOXA2 expression after 4 days of DE differentiation.

See also Figure S1.