Figure 3.

Effects of PSS with rat blood as a perfusate to induce HPV in the isolated perfused/ventilated mouse lung. The blood mixed with perfusate (40 mL PSS and 10 mL whole blood of rat) was used as a perfusate. (A) HPV (1% O₂) was repetitively induced in the absence of a pretone agent. (B) Effects of Ang-II (1 mg/100 ml) as a pretone agent on basal PAP and HPV. After the 3rd hypoxic challenge via ventilator in the isolated lung, the injection of Ang- II was applied into the PAP. Application of angiotensin II induced a transient increase of basal PAP; however, the amplitude of HPV did not significantly increase in the presence of Ang-II. (C) Comparison between before (control) and after AII treatment is shown as a bar graph. Bar graph shows mean ± SEM. Lungs are isolated from eight mice.