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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Nat Med. 2013 May 5;19(6):747–752. doi: 10.1038/nm.3161

Figure 3. Response of TIL 3309 to candidate epitopes identified from autologous tumors.

Figure 3

(a) A screening assay was carried out to evaluate the release of IFN-γ from TIL 3309 in an overnight co-culture with COS-A11 cells that were pulsed individually with the top 46 candidate HLA-A*1101-binding peptides identified from 3309 mel (Supplementary Table 5). Peptides 37-46 (Supplementary Table 5) stimulated the release of less than 100 pg/ml of IFN-γ from TIL 3309 and are not depicted in this graph. The autologous 3309 mel stimulated the release of 4,600 pg/ml of IFN-γ from TIL 3309 in this assay. (b,c) COS7 cells stably transduced with a retroviral vector expressing HLA-A*1101 (COS-A11) were pulsed with the indicated concentrations of peptides in an overnight co-culture with TIL 3309, and IFN-γ release measured. d. COS-A11 cells were transiently transfected with the indicated constructs and evaluated for their ability to stimulate IFN-γ release from TIL 3309 in an overnight co-culture.