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Pyrexia is not required for synchronization to 25 : 29°C TCs. Locomotor behaviour of (a) Canton S, (b) pyx3, (c) pyx3/pyxDf9 and (d) pyxGe pyx3 male flies was analysed in LD, followed by two 25 : 29°C TC in DD as described in the legends to figures 1 and 2. Low and high temperatures are indicated in orange and red, respectively. After TC2, flies were released to constant 25°C in DD. Note that all genotypes show synchronized activity peaks during this temperature interval.
