Table 1.
The sequence of primers accompanied with their annealing temperatures and expected PCR product sizes
| Primer | Primer sequences | Annealing temperature |
Product size (bp) |
|---|---|---|---|
| VEB-F VEB-R |
5'-GAAACAACTTTGACGATTGA-3' 5'-CCCTGTTTTATGAGCAACAA-3' |
50°C | 370 |
| PER1-F PER1-R |
5'-ATGAATGTCATTATAAAAGC-3' 5'-AATTTGGGCTTAGGGCAGAA-3' |
50°C | 925 |
| PER2-F PER2-R |
5'-GTAGTATCAGCCCAATCCCC-3' 5'- CCAATAAAGGCCGTCCATCA-3' |
50°C | 738 |
| INT-1-F INT-1-R |
5’-GGTGTGGCGGGCTTCGTG-3' 5’-GCATCCTCGGTTTTCTGG-3' |
55°C | 456 |
| OXA-51F OXA-51R |
5’-ACAAGCGCTATTTTTATTTCAG-3' 5’-CCCATCCCCAACCACTTTT-3' |
51°C | 641 |