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CD133+ stem cell purification and infection. A: Result of CD133+ cells purification from HCB by flow cytometry. In each case, purification efficiency was above 90%. B: (B, above) Transduced CD133+ cells examined by light microscopy. (B, below Transduced CD133+ cells examined by fluorescent microscopy. Transduction efficiency of CD133+ cells with pCDH-16 and pCDH-451 was obtained approximately more than 70% as determined by fluorescent microscopy
