Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Predicted DNA fragment lengths after digestion of plasmid pUCBM21. Cap2Y444730F DNA with ApaLI, EcoRI, NcoI, SmiI or XhoI. pUCBM21. Cap2Y500730F and pUCBM21.Cap2Y444500730F yield identical restriction pattern as pUCBM21.Cap2Y44730F except for the presence of three instead of two EcoRI cleavage products
| Restriction enzyme | pUCBM21.Cap2Y444730F DNA fragments (bp) |
|---|---|
| ApaLI | 3539, 1246, 497 |
| EcoRI | 2738, 2544 (1712 + 832 in Y500F mutant plasmids) |
| NcoI | 4561, 721 |
| SmiI | 2959, 2323 |
| XhoI | 5096, 186 |
