Fig. 4.
HPLC chromatograms of Bauer ketone 24 across Caco-2 cell monolayers as pure compound or from Echinacea species. A. Bauer ketone 24 standard at 100 μM (retention time 17.6 min). B. Bauer ketone 24 (100 μM) was not detected in the basolateral supernatant fluid, but two more hydrophilic metabolites were detected (retention time 13.3 and 15.4 min). C. Bauer ketone 24 was shown in the basolateral side after apically applied E. sanguinea containing 100 μM of ketone 24 (retention time 17.6 min). D. Bauer ketone 24 was shown in the basolateral side after apically applied E. pallida containing 100 μM of ketone 24 (retention time 17.6 min).