Fig.2. Histone H2A threonine 121 phosphorylation is induced in response to IR-induced DNA damage.
(A) HeLa cells transfected with control or Bub1 siRNA were treated with mock or IR (4Gy). Total cell lysates were collected followed by immunoblotting using indicated antibodies. (B) HeLa cells stably transfected with control shRNA or ATM shRNA were treated with mock or IR (4Gy). Total cell lysates were collected followed by immunoblotting using indicated antibodies.