Fig. 7.

Inducible Hsp70-specific stabilized HIF-1α protein upon arsenite exposure. a The construct expressing inducible Hsp70 and vector construct (as control) were introduced separately into p85α^−/− MEFs, and the corresponding stable transfectant was established. The induction of HIF-1α due to arsenite exposure was determined as indicated in p85α^−/− (Vector), p85α^−/− (Induc Hsp70), and p85α^+/+ MEFs. The expression levels of inducible Hsp70 were verified by Western blotting. b p85α^−/− MEFs were stably transfected with constitutive Hsp70 (Hsc70) expressing construct or the control vector. The success of transfection was confirmed by Western blotting (left panel). The arsenite-induced HIF-1α was detected in both transfectants (right panel). c WT MEFs were stably transfected with nonsense shRNA or two specific shRNAs targeting inducible Hsp70. The efficiency of knockdown was identified by Western blotting and the arsenite-induced HIF-1α protein expression was determined as indicated