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. 2013 Aug 19;69(Pt 9):1001–1003. doi: 10.1107/S1744309113020289

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© Punekar & Selmer 2013

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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(a) Elution profile of RlmJ from a HiLoad 16/60 Superdex 75 gel-filtration column. The major peak corresponds to monomeric RlmJ. Elution volumes of protein molecular-mass standards are indicated at the top. (b) PhastGel 8–25% gradient SDS–PAGE analysis of RlmJ purification. Lane 1, molecular-weight marker (labelled in kDa); lane 2, cell lysate; lane 3, flowthrough from Ni Sepharose column; lane 4, final wash fraction from Ni Sepharose column; lanes 5–7, elution fractions from Ni Sepharose column; lane 8, final concentrated RlmJ after HiLoad 16/60 Superdex 75 gel-filtration column.