Figure 3.

Crystals of the Tn3A–CD40L and Tn3B–CD40L complexes. (a) A crystal of Tn3A–CD40L, which grew to dimensions of 0.15 × 0.15 × 0.1 mm. Conditions were 45 µl reservoir solution (0.2 M NaNO₃, 20% PEG 3350) mixed with 5 µl 0.1 M BaCl₂.2H₂O; 300 nl of this mixture was then combined with 300 nl protein stock solution. (b) Crystals of Tn3B obtained during the initial screening phase using Wizard Cryo 1 and 2 (Emerald BioStructures) under condition F7 (40% PEG 600, 0.1 M sodium acetate, 0.2 M MgCl₂).