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. 2013 Aug 30;8(8):e73143. doi: 10.1371/journal.pone.0073143

Figure 5. TLRs 2 and 9 cooperate in generation of the Th17 response following repeated SR exposures.

Figure 5

WT, TLR2-/-, TLR9-/-, and TLR2/9-/- mice were exposed to SR 3 times / week for 3 weeks and analyzed 4 days after the last exposure. Lung cells were stimulated with SR lysate or media alone for 6 hrs and intracellular cytokine staining performed to measure expression of IL-17A and IL-10 by CD4+/βTcR+ cells. Representative dot plots are shown (n = 3; repeated 2 times) Mean ± SD of CD4+/IL-17A+ WT unexposed = 0 ± 0%; CD4+/IL-17A+ WT exposed = 6.7 ± 2.2%; CD4+/IL-17A+ TLR2-/- exposed = 4.8 ± 0.8%; CD4+/IL-17A+ TLR9-/- exposed = 5.4 ± 1.0%; CD4+/IL-17A+ TLR2/9-/- exposed = *2.5 ± 1.4%. Significance was determined using one-way ANOVA (*p < 0.05 compared to WT SR exposed mice).