Figure 4. Effect of autophagic deficiency on the behavior of chloroplast degradation in mesophyll cells.
A, B, C and D. Mesophyll cells of fresh leaves excised from the CT-GFP transgenic atg5-1 plant (A and B) or CT-GFP plant (C and D) were infiltrated with 10 mM MgCl2 (A) or avirulent Pst DC3000 (AvrRps4) (OD600 = 0.1) (B, C and D) and incubated in 10 mM MES-NaOH (pH 5.5) with the addition of 1 μM CA (A and B) or in 10 mM MES-NaOH (pH 5.5) with the addition of 1 μM CA and 10 μM 3-MA (C and D) at 23 °C for 12 h. D, Magnification of a mesophyll cell of leaves incubated in the conditions described for C, respectively. Chlorophyll fluorescence appears red, and CT-GFP appears green. In merged images, the overlap of GFP and chlorophyll fluorescence appears yellow. Spherical bodies only having GFP (arrows) and whole chloroplast degradative bodies (arrowheads) were observed. Scale bars represent 20 μm.