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. 2013 Aug 30;8(8):e73091. doi: 10.1371/journal.pone.0073091

Figure 6. Expression pattern of related genes in wild-type (WT) and atg5-1 plants.

Figure 6

A. Expression of RPS4, EDS1, PAD4, ATG8a, NPR1, PR1 and RBCS in normal light (N) environment and low light (L) environment of wild-type and atg5-1 plants during the avirulent Pst DC3000 (AvrRps4) treatment. Total RNA was isolated from third and fourth leaves (about 0.1g, collected at 0, 1, 2, 3 and 4 days) of each plant and subjected to semiquantitative RT-PCR using gene-specific primers. 18s ribosomal RNA was used as an internal control. B. Q-PCR quantification of NPR1 and EDS1 mRNA levels in WT (gray), atg5-1 (white) 0 and 2 or 3 d after inoculation with avirulent Pst DC3000 (AvrRps4). Error bars represent SD of the mean and standard deviation of values obtained from three biological samples per genotype and time point. The asterisk indicates a significant difference from control (*, P < 0.05; **, P<0.01).